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We sequenced the genome and transcriptome of 3 male and 3 individuals that are female all the 4 target types

We sequenced the genome and transcriptome of 3 male and 3 individuals that are female all the 4 target types

Outcomes and Discussion

(P. Wingei, P. Picta, Poecilia latipinna, and Gambusia holbrooki) (SI Appendix, Table S1) selected to express a also taxonomic circulation across Poeciliidae. For each species, we created DNA sequencing (DNA-seq) with on average 222 million 150-base set (bp) paired-end reads (average insert size of 500 bp, leading to on average 76-fold protection) and 77.8 million 150-bp mate-pair reads (average insert measurements of 2 kb, averaging 22-fold protection) per individual. We additionally created, an average of, 26.6 million 75-bp paired-end RNA-seq checks out for each individual.

Past focus on the intercourse chromosomes of those types showed proof for male heterogametic systems in P. Wingei (48), P. Picta (50), and G. Holbrooki (51), and a lady system that is heterogametic P. Latipinna (52, 53). For every target types, we built a scaffold-level de novo genome construction using SOAPdenovo2 (54) (SI Appendix, Table S2). Each installation had been built utilising the reads through the homogametic intercourse just to be able to avoid coassembly of X and Y reads. Continue reading We sequenced the genome and transcriptome of 3 male and 3 individuals that are female all the 4 target types